Relationship between the level of reactive oxygen species in native rooster spermatozoa and the quality indicators of fresh and frozen-thawed sperm

Purpose: to study the influence of level active forms oxygen in native spermatozoa roosters on qualitative indicators freshly exposed and deconed individual ejaculators (mobility, damage cell membranes sperm) determining permissible AFC generation improve cryoponement protocol. Materials methods. The object was Rodi-Aland Red (n = 20) breed at age 44 weeks life. All males were kept cells with “Genetic Collection Rare Disappearing Course breeds” VNIIGRZH systems adopted by BRK for feeding, posting light regime. Sperm received penicillin bottles a volume 10 ml, using abdominal massage method. They measured each ejaculate, assessed mobility sperm, concentration. Cryoconservational carried out granules. Thawing granules T 60 ° C slit tie. plasma sperm seed evaluated Suppitial Bluma coloring Spermatozoa damaged painted red, intact remained white (colorless). Each drug estimates least 200 cells. To determine levels roosters, method based luminol-proroxidate hemilyuminescence used, which chemilyuminometer Lum-1200. time measurement 3 hours, hemiluminiscence form (given growth indicator, peak decline). Cell concentration (7x106 classes/ml) selected experimentally measurements, according results series preliminary experiments. Results. As result study, data chemilyuminescence first obtained. range (from 75 249 volts*sec) has been established, do not receive significant membrane structures during cryoponservation. In case exceeding threshold 250 volts*S, number increases sharply from 17.19% 62.87%. obtained allow assessment selection quality their purposes cryoponservation formation cryobans reproductive